MICANS PharmB, PHIL
The National Institute on Aging (NIA) announced the final results of testing from three government labs regarding the
patented antioxidant nordihydroguaiaretic acid (NDGA). All three labs agreed that NDGA extended lifespan by a
resounding 12% in mice (1) (see Figure 1).
When some read this astounding news, they were skeptical. With a note of cynicism and doubt in their voices, they
said this report was probably hyperbole and the Federal government is not to be trusted. Yet these same three
government labs had also conducted lifespan studies with much-hyped anti-aging remedies, resveratrol, curcumin,
green tea, oxaloacetic acid and triglyceride oil (2) and found that these five supplements did not extend lifespan in
During the 1980s, researchers extensively tested NDGA in humans, mice and dogs. Results indicated that NDGA
extended lifespan in a variety of mammals. Even the US Patent and Trademark Office approved these results and
granted a patent. This office granted Dr. Richard Lippman a patent for NDGA, as part of a formula developed to slow
aging and extend human lifespan based on his extensive and convincing NDGA research (3).
Background Study of NDGA
Before Dr. Lippman was awarded a US patent on NDGA, several attorneys voiced skepticism. In firm language, they
stated that every law school student knows that two types of patents are never granted: a patent on a perpetual
motion machine and a patent on a fountain-of-youth remedy. Apparently, Dr. Lippman convinced patent examiners
that his clinical human, mice and dog studies of NDGA were sufficient to warrant a patent with claims to retard human
aging. These studies were also sufficient for the drug licensing authorities of Sweden and Italy to grant Dr. Lippman
marketing rights to sell NDGA under the name ‘Aging Control Formula 228’ (ACF228®).
Interestingly, a prominent American businessman, A. Glenn Braswell, had heard Dr. Lippman’s story, but Braswell
doubted that it was sold at the Vatican pharmacy in Rome, Italy. Consequently, he took his wife on a sudden trip to
Rome—and, to his surprise, found that ACF228 was indeed sold at the Vatican pharmacy with the pope’s blessings!
ACF228® Is Based on Extensive Free Radical Research
Today, we know free radicals are not antiwar activists out on bail. But when Dr. Richard Lippman was doing research
in Sweden many years ago, most people thought the term ‘free radicals’ referred to some kind of hippie politics.
No one then knew about these molecular sharks’ devastating effects on the human body and their role in aging.
Indeed, only twenty-five years ago, free radical chemistry and the toxic effects of free radicals on the human body
were unknown to most of the general public and even to many doctors and medical researchers.
Dr. Lippman first learned about the free radical theory of aging as an undergraduate student. When he began doing
graduate research work in cell biology, he and his colleagues held conferences at Pharmacia-Upjohn and the
University of Uppsala to discuss the exciting findings of Professor Denham Harman, whose experimental work at the
University of Nebraska in the 1950s showed that the life spans of mice could be extended 50%
with special antioxidant supplementation. The press and public responded; “So what?”
However, Sweden is well known in science and engineering for its industrial and technical advances. And Lippman
was the leader of a large medical staff that encouraged progressive research.
Raising Funds for Research
Dr. Lippman wanted to take Harman’s work one-step further and explore the relationship between free radicals and
aging. He turned to Professor Sven Brolin, chair of the University of Uppsala’s Department of Medical Cell Biology
and Professor Gunnar Wettermark, chair of the Royal Institute of Technology’s Department of Physical Chemistry, for
assistance in raising funds for research.
Dr. Lippman was successful, receiving significant medical and chemical grants from the Swedish Research Council to
develop antiaging strategies based on Harman’s groundbreaking discovery of the action of free radicals and the role
of radical scavengers (antioxidants) in destroying or inhibiting them. The Swedish Research Council financed years of
Dr. Lippman’s research at the Royal Institute of Technology in Stockholm and at the University of Uppsala,
Scandinavia’s oldest university, which has an anatomy lecture hall built in the 15th century.
Dr. Lippman’s research into the role that free radicals play in the breakdown of the aging body led him to develop one
of the most potent antioxidant combinations yet known, a unique antioxidant cocktail containing NDGA and called
No Typical Scientist
Dr. Lippman’s normal lab attire—jeans, a khaki shirt, and ostrich leather boots—breaks from the conventional notion
of a white-coated scientist. Before his work in antiaging research that made him famous, he ate junk food. Now, a
typical lunch for him is salmon sashimi and salad or bi bim bop with a bowl of miso soup. He even developed his own
recipe for sugar-free, gluten-free, walnut cinnamon pumpkin muffins.
In speaking, Dr. Lippman presents an easy smile and laugh. He may not look like a typical scientist, but his passion
for longevity research is real. His innovative research into free radical pathology helped put antioxidants on the map,
in the dictionary and in the supermarket.
Once funding was in place, Dr. Lippman gathered a team of five prominent Swedish scientists to help him develop
methods for measuring free radicals and biochemical changes related to aging: Professor Agneta Nilsson, a
nutritionist and alternative medical professional with advanced degrees in nursing and teaching; Dr. Ambjörn Ågren,
MD, PhD, who had received numerous awards in the field of emergency medicine; Professor Mathius Uhlén, PhD, a
civil engineer, molecular biologist, and, later, professor and chair of the Royal Institute of Technology’s Department
Molecular Biology; Evald Koitsalu, an engineer and expert in computer hardware and software; and Dr. Kaj
Alverstrand, a psychologist and consultant to Volvo.
With these tremendous financial and personnel resources, Dr. Lippman was able to achieve great leaps in the field of
antiaging. Indeed, Paul Glenn of the Paul Glenn Foundation for Antiaging Research said that Dr. Lippman’s work
was; “light years ahead of everyone else!”
Dr. Lippman’s research resulted in a patent for NDGA and a product that promotes better health and longevity:
Cellular Model—A Better Choice
The research team’s first task was to find a cellular model rather than an animal model to test for life extension, since
the Harman model of waiting for mice to grow old and die was costly and took years of patience before the results
The Lippman team had access to many different types of living cells in culture, such as human cells of the heart,
brain, liver and central nervous system. In 1980, Lippman invented special probes that would penetrate cell interiors
without harming them. For the first time in the history of cell biology, scientists were able to measure free radicals in
living cells (4). The first probe, carnitinylmaleate luminol (CML), measured superoxide radicals in live human liver
cells. Dr. Lippman and his team went on to test many different combinations of antiaging nutrients.
Developing the formula combinations was a tedious process. Live cells were harvested from biopsies, then separated
and kept metabolically alive in special culture dishes heated to a constant 98°F. The live cells were removed as
needed by the research team and tested for their health by means such as measurements of adenosine triphosphate
(ATP), the power source or ‘gasoline’ of most cell activities.
Then the cell cultures were impregnated with special CML probes and incubated with different mixtures of vitamins
and known antiaging nutrients. Lippman’s team eventually tested 227 different mixtures to find an optimal mixture
with pronounced longevity-promoting characteristics. Mixture number 228 was found to work best, and this and
several other promising mixtures such as 223 were tested further in mice and human volunteers. Now named
ACF228®, the mixture proved successful in extending mice health and life spans, (see Figure 2)
Scientific Community Astounded
The team published its results in more than twenty prominent medical journals. The work astounded the Swedish
scientific community and Lippman was nominated for a Nobel Prize in Medicine in 1996.
Further tests were conducted on hundreds of human volunteers recruited from several Swedish hospitals (3). The
volunteers were tested to establish their normal levels of fatty-acid peroxides, which are free-radical downstream
products, and then were fed varying amounts of ACF228® and other nutrients. Once again, the mixture known as
ACF228® caused normal peroxide levels to decline the fastest. Lippman and his researchers performed other human
tests that indicated ACF228® also had beneficial effects on the skin and sexual function (3).
“We found that the ACF228® formula truly is beneficial,” Dr. Lippman says. “It was especially helpful for middle-aged
and older people; their liver function became like that of teenagers. Often people experience reduced liver function as
they age, especially if they have abused their bodies with heavy
consumption of alcohol and a high sugar diet, causing metabolic syndrome (5). This nutrient mix offers protection
from a multitude of free radicals in the body.”
Ultimately, the ACF228® formula was approved for use by regulatory agencies in both Sweden and Italy and then
patented in the United States.
Indeed, based on these criteria, Dr. Lippman could rest easy. But he isn’t resting. The energetic, youthful-looking
father of three sons and four grandchildren still goes to his lab daily. And what is this Nobel Prize nominee working on
today for the betterment of humankind tomorrow? Dr. Lippman continues his medical research at the behest of
International Antiaging Systems, focusing on improved methods of delivering important vitamins and hormones via
transdermal patches and creams. “Failure to absorb nutrients is a tremendous problem, and 80% of Americans have
problems swallowing pills and capsules,” he says (5).
“The response to ACF228® worldwide has been enormous,” says Dr. Lippman; “that it is indeed gratifying. You know,
we should all be able to live to 120 years and perhaps even beyond. We don’t because of the free radical damage
and declining repair hormones our cellular systems sustain. Our brains shrink, our arteries become hardened and our
liver function declines, mostly because of free radical pathology and damaged endocrine glands. Aging is the ultimate
disease; if ACF228, with its unique blend of natural ingredients can help people to prevent their premature onset,
then I will have lived my life knowing that it has been a success.”
1. Strong, R et. al., Oct. 2008, Aging Cell, 7(5), pp. 641-650.
2. Strong, R et. al, Jan 2013, J Gerontology, 68(1), pp. 6-16.
3. Harman, D., Jul. 1956, J Gerontology, 11(3), pp. 298-300.
4. Lippman, R, 1987, US Patent No. 4,695,590.
5. Lippman, R, 1980, Experimental Gerontology, vol. 20, pp. 46-52.
5. Lippman, R. 2009, Stay 40, Outskirts Press Inc., Boulder, Colorado.